GSM2476258: MCF7 H3K4me2 G0/G1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7 breast cancer cell line
cell line
MCF7
cell type
breast cancer
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
GRO-seq: Nuclear run-on RNA was further purified with TURBO DNA-freeTM kit (Life Technologies, AM1907) to remove residue DNA contamination. RNA-seq: Total RNA was extracted from synchronized MCF-7 cells with TRIZOL REAGENT (Life Technologies, 15596026) and fragmentized with sonication. ChIP-seq: Chromatin from synchronized MCF-7 cells was digested with Nuclease micrococcal from Staphylococcus aureus (Sigma Aldrich, N3755-50UN) and dialyzed with Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher Scientific, PI66380). Dynabeads Protein A (Life Technologies, 10002D) and G (Life Technologies, 10004D) were used for immunoprecipitation with antibodies against H3K4me2 (EMD Millipore, 07-030) and H3K27ac (Abcam, ab4729). GRO-seq and RNA-seq libraries were constructed with Encore Complete RNA-Seq DR Multiplex System 1-8 (Nugen Technologies lnc, 0333-32). ChIP-seq libraries were constructed with ThruPLEX DNA-seq kit (Rubicon) and sequenced to 50bp with Illumina HiSeq machine.