Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A
chip antibody
Histone 3 K27 Acetylation - (ab4729, abcam)
treatment
dexamethasone (DEX)
treatemtn time
240 min

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell fixation and chromatin immunoprecipitation were performed as described (Blecher-Gonen et al., 2013) Library construction and sequencing were performed as described (Blecher-Gonen et al., 2013)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
14700944
Reads aligned (%)
97.1
Duplicates removed (%)
2.7
Number of peaks
15885 (qval < 1E-05)

hg19

Number of total reads
14700944
Reads aligned (%)
96.8
Duplicates removed (%)
3.0
Number of peaks
15907 (qval < 1E-05)

Base call quality data from DBCLS SRA