GSM2473263: rWT H3K27ac ChIP replicate1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
RAJI
Primary Tissue
Blood
Tissue Diagnosis
Malignant Lymphoma - Burkitts Type
Attributes by original data submitter
Sample
source_name
Raji B cells
chip antibody
H3K27ac (Abcam, ab4729, lot GR238071-1)
genotype
rWT
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested at 72 hours after induction (48 hours after α-amanitin) to perform ChIP using specific antibodies or total RNA, polyA-RNA and Nascent RNA was extracted using corresponding protocols as described in manuscript. ChIP-seq libraries were prepared with Illumina's ChIP Sample Prep Kit according to standard protocol. RNA-seq Libraries were prepared either with Illumina's Small RNA Library Prep Kit by using a modified protocol described in manuscript or with Epicenter's ScriptSeq V2 library preparation protocol according to manufacturer's instructions.