Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Blood

Cell type

RAJI

Primary Tissue

Blood

Tissue Diagnosis

Malignant Lymphoma - Burkitts Type

Sample

source_name

Raji B cells

chip antibody

HA (Abcam, ab9110, lot GR235874-8)

genotype

YFFF

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were harvested at 72 hours after induction (48 hours after α-amanitin) to perform ChIP using specific antibodies or total RNA, polyA-RNA and Nascent RNA was extracted using corresponding protocols as described in manuscript. ChIP-seq libraries were prepared with Illumina's ChIP Sample Prep Kit according to standard protocol. RNA-seq Libraries were prepared either with Illumina's Small RNA Library Prep Kit by using a modified protocol described in manuscript or with Epicenter's ScriptSeq V2 library preparation protocol according to manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

43539900

Reads aligned (%)

98.3

Duplicates removed (%)

71.1

Number of peaks

728 (qval < 1E-05)

hg19

Number of total reads

43539900

Reads aligned (%)

97.4

Duplicates removed (%)

71.9

Number of peaks

635 (qval < 1E-05)