Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESCs_Trim28 shRNA_input

strain background

12910la

cell line

J1

cell type

mouse embryonic stem cells (mESCs)

transduced with

lentiviral vector encoding for shRNA targeting Trim28

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was prepared from 10 million cells as previously described (Rowe et al., 2013b). Libraries were ligated with Illumina adaptors

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

29107815

Reads aligned (%)

96.5

Duplicates removed (%)

15.0

Number of peaks

383 (qval < 1E-05)

mm9

Number of total reads

29107815

Reads aligned (%)

96.4

Duplicates removed (%)

15.1

Number of peaks

346 (qval < 1E-05)