Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mESCs_control shRNA_input
strain background
12910la
cell line
J1
cell type
mouse embryonic stem cells (mESCs)
transduced with
lentiviral vector encoding for a control shRNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared from 10 million cells as previously described (Rowe et al., 2013b). Libraries were ligated with Illumina adaptors

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
27515742
Reads aligned (%)
97.0
Duplicates removed (%)
14.9
Number of peaks
363 (qval < 1E-05)

mm9

Number of total reads
27515742
Reads aligned (%)
96.8
Duplicates removed (%)
15.0
Number of peaks
317 (qval < 1E-05)

Base call quality data from DBCLS SRA