Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

source_name

H1 human embryonic stem cells

source

H1 human embryonic stem cells (hESCs)

transfected with

plasmid encoding for a HA-tagged form of DUX4

time point

24 hours after transfection

chip antibody

HA.11 (BioLegend, 901502)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Libraries were ligated with Illumina adaptors Chromatin was prepared from 10 million cells as previously described (Rowe et al., 2013b).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

44411505

Reads aligned (%)

94.3

Duplicates removed (%)

4.5

Number of peaks

62114 (qval < 1E-05)

hg19

Number of total reads

44411505

Reads aligned (%)

93.4

Duplicates removed (%)

5.1

Number of peaks

63045 (qval < 1E-05)