Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Bone

Cell type

A-673

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

Rhabdomyosarcoma

Sample

source_name

A673

chip antibody

HA (ROCHE #11867423001)

source_type

cell line

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin from formaldehyde-fixed cells (2-5 x 10^6 cells per epitope) was fragmented to a size range of 200 - 700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with the indicated antibodies and Protein G-Dynabeads (Life Technologies). Immunoprecipitated DNA was extracted with AMP Pure beads(Beckman Coulter) after crosslink reversal, RNAse A and Proteinase K treatment. ChIP DNA samples were used to generate Illumina sequencing libraries following standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

27679966

Reads aligned (%)

97.7

Duplicates removed (%)

39.4

Number of peaks

740 (qval < 1E-05)

hg19

Number of total reads

27679966

Reads aligned (%)

96.7

Duplicates removed (%)

40.6

Number of peaks

710 (qval < 1E-05)