Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Brd4

Cell type

Cell type Class
Blood
Cell type
Lymphoma cell lines
NA
NA

Attributes by original data submitter

Sample

source_name
Eμ-Myc lymphoma #1 cell line
cell line
E<mu>-Myc lymphoma #1 cell line
treatment
DMSO vehicle
culture media
High-glucose Dulbecco's modified Eagle medium supplemented with 10% fetal calf serum, penicillin (100 U/mL), streptomycin (100mg/mL), 0.1mM L-Asparagine, and 50μM 2-mercaptoethanol
chip antibody
anti-Brd4 (ab128874, Abcam)
strain
C57BL/6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehyde cross-linked cells and sonicated to release the chromatin, then immunoprecipitated using relevant antibodies. For BRD4, RNA Polymerase II, IRF1, STAT1, H3K27Ac ChIP-Seq, indexed libraries were prepared using KAPA Hyper Prep Kit for Illumina platforms (Kapa Biosystems) and SeqCap Adapter Kit (Roche). For MYC ChIP-Seq, indexed libraries were prepared using Nugen Ovation Ultralow system (v2, Nugen protocol M01379v3).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
17914999
Reads aligned (%)
92.7
Duplicates removed (%)
6.9
Number of peaks
6315 (qval < 1E-05)

mm9

Number of total reads
17914999
Reads aligned (%)
92.5
Duplicates removed (%)
7.0
Number of peaks
6318 (qval < 1E-05)

Base call quality data from DBCLS SRA