Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
msl-2

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 cells
cell line
S2
gender
male
chip antibody
anti-MSL2 (Becker lab)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments (Covaris) were performed according to (Schauer et al., 2013) adapted to cell lines (Villa et al., 2016). Briefly, cells were re-suspended in ice-cold homogenization buffer and fixed with 1% formaldehyde for 10 minutes at room temperature. After quenching with 125 mM glycine, the cells were collected, washed and sonicated in RIPA buffer with a Covaris sonifier (PIP: 140, DF 20%, CB: 200) for 30 min. MSL2 antibody was previously described in (Villa et al., 2012) and H4K16ac was acquired from Active Motif (#39167).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

dm3

Number of total reads
31310321
Reads aligned (%)
95.8
Duplicates removed (%)
32.3
Number of peaks
7832 (qval < 1E-05)

dm6

Number of total reads
31310321
Reads aligned (%)
95.6
Duplicates removed (%)
33.9
Number of peaks
6614 (qval < 1E-05)

Base call quality data from DBCLS SRA