Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
S2 cells
cell line
S2
gender
male
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments (Covaris) were performed according to (Schauer et al., 2013) adapted to cell lines (Villa et al., 2016). Briefly, cells were re-suspended in ice-cold homogenization buffer and fixed with 1% formaldehyde for 10 minutes at room temperature. After quenching with 125 mM glycine, the cells were collected, washed and sonicated in RIPA buffer with a Covaris sonifier (PIP: 140, DF 20%, CB: 200) for 30 min. MSL2 antibody was previously described in (Villa et al., 2012) and H4K16ac was acquired from Active Motif (#39167).

Platform Information


instrument_model
Illumina HiSeq 1500

External Database Query

Logs in read processing pipeline


Number of total reads
18939179
Reads aligned (%)
97.7
Duplicates removed (%)
13.6
Number of peaks
3300 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA