Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A
cell line
MCF10A
chip antibody
Rpb1 NTD - 14958S cell signaling

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell fixation and chromatin immunoprecipitation were performed as described (Blecher-Gonen et al., 2013) Library construction and sequencing were performed as described (Blecher-Gonen et al., 2013)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
17102444
Reads aligned (%)
98.1
Duplicates removed (%)
19.6
Number of peaks
12378 (qval < 1E-05)

hg19

Number of total reads
17102444
Reads aligned (%)
97.7
Duplicates removed (%)
20.1
Number of peaks
12392 (qval < 1E-05)

Base call quality data from DBCLS SRA