GSM2467359: Input replicate 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
HeLa (Kyoto), Input
cell line
HeLa (Kyoto)
transfected plasmid
pIRESneo-EGFP
chip antibody
GFP (Abcam, ab290, lot GR278073-1)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HeLa Kyoto cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Nuclear lysates were subjected to chromatin shearing to an average size of 150 bp. Histone-DNA complexes were isolated with GFP-antibody. All libraries were prepared with the MicroPlex library preparation kit v2 (order no C05010012) following manufacturer's instructions.