Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293T
gender
male
tissue
Kidney
chip antibody
anti-human RUNX1 antibody (Abcam, cat.no ab23980)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and RUNX1 or TET2-DNA complexes were isolated with antibody. ChIPed DNA was subjected to the library construction with KAPA Library Preparation Kit (KAPA Biosystems, Imc.,MA, USA) according to the manufacture’s instruction.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
43396022
Reads aligned (%)
75.6
Duplicates removed (%)
13.8
Number of peaks
104608 (qval < 1E-05)

hg38

Number of total reads
43396022
Reads aligned (%)
77.5
Duplicates removed (%)
12.4
Number of peaks
105517 (qval < 1E-05)

Base call quality data from DBCLS SRA