Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXA1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 breast cancer cells
cell line
MCF7
chip antibody
FOXA1 antibodies mixed ab5089, ab23738
induction
ShCtrl

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, lysed in a buffer containing SDS and sonicated. ThruPLEX-FD Prep kit, Rubicon Genomics

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
52821144
Reads aligned (%)
78.9
Duplicates removed (%)
9.3
Number of peaks
8267 (qval < 1E-05)

hg38

Number of total reads
52821144
Reads aligned (%)
83.4
Duplicates removed (%)
5.9
Number of peaks
9232 (qval < 1E-05)

Base call quality data from DBCLS SRA