Antigen

Antigen Class

TFs and others

Antigen

FOXA1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF-7 breast cancer cells

cell line

MCF7

chip antibody

FOXA1 antibodies mixed ab5089, ab23738

induction

ShCtrl

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked, lysed in a buffer containing SDS and sonicated. ThruPLEX-FD Prep kit, Rubicon Genomics

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

52821144

Reads aligned (%)

78.9

Duplicates removed (%)

9.3

Number of peaks

8267 (qval < 1E-05)

hg38

Number of total reads

52821144

Reads aligned (%)

83.4

Duplicates removed (%)

5.9

Number of peaks

9232 (qval < 1E-05)