GSM2466423: control H3K27ac ChIP-seq rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
control HL-60 cells
cell line
HL-60; ATCC CCL-240
tissue origin
peripheral blood
disease
acute promyelocytic leukemia
treated with
ethanol for 4 days
chip antibody
H3K27ac (abcam, ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were treated with 1% formaldehyde to crosslink. Then cell membrane were broken using lysis buffer (50mM HEPES-KOH, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1% SDS). Chromatin were resuspended using FA lysis buffer (50mM HEPES-KOH, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS) and fragmented by ultra-sonication processor (cole-parmer, USA). Immunoprecipitation were performed overnight using dynabeads (Thermo Fisher) pre-incubated with anti-H3K4me3 and H3K27ac antibodies (abcam, England). Library construction was performed using TruePrep DNA Library Prep Kit (Vazyme, China) according to the manufacture's protocol.