Antigen

Antigen Class

TFs and others

Antigen

SREBF2

Cell type

Cell type Class

Pancreas

Cell type

PANC-1

Tissue

Pancreas/Duct

Disease

Epithelioid Carcinoma

Sample

source_name

PANC-1 low pH SREBP2 ChIP

culture condition

low pH (pH 6.8)

ChIP

SREBP2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA or SREBP2-DNA complexes were isolated. For FAIRE-seq, sonicated nuclear fructions were subjected to phenol/chloroform extraction twice followed by ethanol precipitation. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

34389028

Reads aligned (%)

96.9

Duplicates removed (%)

8.2

Number of peaks

1050 (qval < 1E-05)

hg19

Number of total reads

34389028

Reads aligned (%)

95.8

Duplicates removed (%)

9.8

Number of peaks

1087 (qval < 1E-05)