Antigen

Antigen Class

TFs and others

Antigen

SREBF2

Cell type

Cell type Class

Pancreas

Cell type

PANC-1

Tissue

Pancreas/Duct

Disease

Epithelioid Carcinoma

Sample

source_name

PANC-1 control SREBP2 ChIP

culture condition

Control (pH 7.4)

ChIP

SREBP2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA or SREBP2-DNA complexes were isolated. For FAIRE-seq, sonicated nuclear fructions were subjected to phenol/chloroform extraction twice followed by ethanol precipitation. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

37462865

Reads aligned (%)

96.3

Duplicates removed (%)

9.3

Number of peaks

977 (qval < 1E-05)

hg19

Number of total reads

37462865

Reads aligned (%)

95.5

Duplicates removed (%)

10.8

Number of peaks

1070 (qval < 1E-05)