Cells were crosslinked at RT by adding 16% paraformaldehyde (Electron Microscopy Sciences 130218) to a final concentration of 1% in 37°C media for 10 or 15 min. Crosslinking was quenched with Tris pH 7.6 to a final concentration of 1.33 M. ChIP protocols vary between experiments and are detailed in the STAR Methods NEXTflex Rapid DNA-Seq Kit (Bioo Scientific 5144-04)