GSM2465105: C-NELF-Flavo-PJ34-0-H2O2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NELFA
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Adherent HeLa cells
tissue
Adherent HeLa cells
treatment
40 min 1 µM flavopiridol, then 20 min 20 µM PJ34
antibody
NELF-A (A-20) (SCBT sc-23599)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked at RT by adding 16% paraformaldehyde (Electron Microscopy Sciences 130218) to a final concentration of 1% in 37°C media for 10 or 15 min. Crosslinking was quenched with Tris pH 7.6 to a final concentration of 1.33 M. ChIP protocols vary between experiments and are detailed in the STAR Methods NEXTflex Rapid DNA-Seq Kit (Bioo Scientific 5144-04)