Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NELFA

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Adherent HeLa cells
tissue
Adherent HeLa cells
treatment
1 h 1 µM flavopiridol
antibody
NELF-A (A-20) (SCBT sc-23599)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked at RT by adding 16% paraformaldehyde (Electron Microscopy Sciences 130218) to a final concentration of 1% in 37°C media for 10 or 15 min. Crosslinking was quenched with Tris pH 7.6 to a final concentration of 1.33 M. ChIP protocols vary between experiments and are detailed in the STAR Methods NEXTflex Rapid DNA-Seq Kit (Bioo Scientific 5144-04)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
13727939
Reads aligned (%)
190.9
Duplicates removed (%)
0.8
Number of peaks
7490 (qval < 1E-05)

hg38

Number of total reads
13727939
Reads aligned (%)
192.9
Duplicates removed (%)
0.7
Number of peaks
7941 (qval < 1E-05)

Base call quality data from DBCLS SRA