Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7

cell line

MCF7

biomaterial provider

ATCC

Sex

female

tissue

Breast

antibody

HC-20 and F10 (Santa Cruz Biotechnology)

treatment

Oleic Acid

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIPseq libraries were prepared with the Hyper Library Construction Kit from Kapa Biosystems with two modifications: adaptors were diluted 1:20 and adaptored DNA were amplified for 6 cycles

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

21997069

Reads aligned (%)

95.7

Duplicates removed (%)

2.3

Number of peaks

3657 (qval < 1E-05)

hg19

Number of total reads

21997069

Reads aligned (%)

95.2

Duplicates removed (%)

3.0

Number of peaks

3682 (qval < 1E-05)