Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MTOR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP human prostate cancer cell line treated with vehicle for 48h
cell line
LNCaP prostate tumor cell line
treatment
control
chip antibody
mTOR

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After 48h treatment with vehicle or R1881 nuclei were isolated and subjected to chromatin immunoprecipitation. Following mTOR ChIP, genomic DNA was purified with QIAGEN DNA extraction kit. Libraries were performed at the Génome Québec Innovation Centre (McGill University) using standard protocol for HiSeq - Illumina Single reads 50bp sequencing lane

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
77104580
Reads aligned (%)
88.5
Duplicates removed (%)
8.5
Number of peaks
91568 (qval < 1E-05)

hg38

Number of total reads
77104580
Reads aligned (%)
91.3
Duplicates removed (%)
6.5
Number of peaks
87895 (qval < 1E-05)

Base call quality data from DBCLS SRA