Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adult
Cell type
Testis
NA
NA

Attributes by original data submitter

Sample

source_name
somatic gonadal cells in fly testis
strain
Tj-Gal4/ UAS-E(Pc) cDNA-GFP; UAS-E(Pc)shRNA/+
developmental stage
adult
age
5 days old
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
[ChIP-seq] Sonication of fly testes was performed. Then sonicated chromatin was incubated with GFP antibody. [ChIP-seq] Libraries were generated using reagents provided in the Illumina TruSeq ChIP Sample Preparation Kit (IP-202-1012). [RNA-seq] Total RNA was purified following the manufacturer’s instruction of PicoPure RNA isolation kit (KIT0204, Life technologies) [RNA-seq] libraries were generated using reagents provided in Illumina TruSeq RNA Sample Preparation Kit (RS-122-2001).

Sequencing Platform

instrument_model
Illumina MiSeq

dm6

Number of total reads
1489562
Reads aligned (%)
99.8
Duplicates removed (%)
0.2
Number of peaks
81 (qval < 1E-05)

dm3

Number of total reads
1489562
Reads aligned (%)
100.0
Duplicates removed (%)
0.1
Number of peaks
125 (qval < 1E-05)

Base call quality data from DBCLS SRA