GSM2459096: AATF ChIPSeq NALM; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AATF
Cell type
Cell type Class
Blood
Cell type
NALM-6
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
B-Cell
cell line
NALM-6
cell type
B-cell
chip antibody
anti-Che-1 (Bethyl, A301-032A)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Extract protocol not provided. Chromatin immunoprecipitations were performed as previously described (Bruno et al, 2002) by using rabbit polyclonal antibodies ChIP grade, anti-Che-1 (Bethyl), and anti c-Myc and Dynabeads Protein G (Life Technologies). The quantity of the immunoprecipitated material was determined by Qubit 2.0 fluorometer (Life Technologies). 10 ng of the immunoprecipitated chromatin was used to prepare the libraries for sequencing following the manufacturer’s instructions including DNA and repairing, adaptor ligation, and amplification (Illumina). The libraries were then sequenced using the Illumina NextSeq 500 system.