Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAL1

Cell type

Cell type Class
Blood
Cell type
Erythroid Precursor Cells
MeSH Description
The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors.

Attributes by original data submitter

Sample

source_name
Erythroid precursor cells
tissue
peripheral blood
cell type
CD34+ HSPC derived erythroid precursors
culture days
6 days expansion followed by 6 days differentiation
chip antibody
TAL1 (Santa Cruz, sc-12984)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Transcription factor-DNA and histone-DNA complexes were isolated from sonicated crosslinked chromatin by antibody precipitation. Libraries were generated using the ChIP-seq Sample Preparation Kit (Illumina) following manufacturer instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
220047767
Reads aligned (%)
20.6
Duplicates removed (%)
42.6
Number of peaks
13841 (qval < 1E-05)

hg38

Number of total reads
220047767
Reads aligned (%)
21.2
Duplicates removed (%)
41.6
Number of peaks
14954 (qval < 1E-05)

Base call quality data from DBCLS SRA