Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
SKNO-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
SKNO1 cells
fusion protein
AML-ETO fusion
cell line
SKNO1
cell type
AML cell line
chip antibody
H3K27Ac antibody (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
51666157
Reads aligned (%)
68.1
Duplicates removed (%)
17.8
Number of peaks
11157 (qval < 1E-05)

hg19

Number of total reads
51666157
Reads aligned (%)
67.7
Duplicates removed (%)
19.0
Number of peaks
11137 (qval < 1E-05)

Base call quality data from DBCLS SRA