Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
Breast cancer cells
NA
NA

Attributes by original data submitter

Sample

source_name
Breast tumor xenograft
xenograft
UCD4
treatment
estrogen plus progesterone
chip antibody
ER (sc-543X, Santa Cruz Biotechnology), lot# F1215

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was pulverized and crosslinked. Lysates were produced from sonicated nuclei and ER or PR were isolated with antibody. Libraries were prepared at the University of Colorado Denver Genomics and Microarray Core facility using the NuGEN Ovation Ultralow Library System v2 .

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
17458074
Reads aligned (%)
67.7
Duplicates removed (%)
23.6
Number of peaks
3005 (qval < 1E-05)

hg38

Number of total reads
17458074
Reads aligned (%)
68.7
Duplicates removed (%)
23.0
Number of peaks
3129 (qval < 1E-05)

Base call quality data from DBCLS SRA