Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Human Endothlial cell line
cell type
Primary Human Umbilical Vein Endothelial Cells
passages
P3-6
antibody
Brd4, Bethyl Laboratories, A301-985A50

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChiP-seq: Chromatin DNA was extracted from sonicated nuclei and further selectively accumulated by ChiP-specific antibody. RNA-seq: total RNA were purified by Qiagen RNeasy column. Illumina multiplex library protocol for ChiP-seq and mRNA seq of VEGF stimualtion is as described (Bing Zhang, et al. , 2013,Genome Res. 2013 Jun;23(6):917-27); Spike-in mRNA-seq protocol followed ScriptSeq v2 RNA-seq kit (Illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
15590764
Reads aligned (%)
88.6
Duplicates removed (%)
8.9
Number of peaks
1227 (qval < 1E-05)

hg38

Number of total reads
15590764
Reads aligned (%)
90.4
Duplicates removed (%)
7.9
Number of peaks
1236 (qval < 1E-05)

Base call quality data from DBCLS SRA