GSM2434433: input DNA FF rep2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Gonad
Cell type
Germline stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
GSCs
tissue
germline stem cells (GSCs)
genotype
Kmt2bF/F
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were crosslinked and chromatin was prepared according to a previous report (Nat. Protoc. 3, 1032–1045) with modifications. For RNA-seq, total RNA was purified using Isogen (Nippon Gene), and treated with DNaseI to digest genomic DNA. ChIP-seq and RNA-seq libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), respectively, according to manufacturer's instructions.