GSM2432984: D283 shNEUROD1.1579 NEUROD1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NEUROD1
Cell type
Cell type Class
Neural
Cell type
D283 Med
Primary Tissue
Brain
Tissue Diagnosis
Medulloblastoma
Attributes by original data submitter
Sample
source_name
NEUROD1 ChIP-seq in D283 cell line infected with shNEUROD1.1579
cell line
D283
chip antibody
NEUROD1
source type
cell line
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from formaldehyde-fixed cells (3-5 x 10^6 cells per epitope) was fragmented to a size range of 200 - 700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with the indicated antibodies and Protein G-Dynabeads (Life Technologies). Immunoprecipitated DNA was extracted with AMP Pure beads(Beckman Coulter) after crosslink reversal, RNAse A and Proteinase K treatment. ChIP DNA samples were used to generate Illumina sequencing libraries following standard protocols.