GSM2432971: D341 CRISPR.OTX2.2 OTX2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
OTX2
Cell type
Cell type Class
Neural
Cell type
D341 Med
Primary Tissue
Brain: Cerebellum
Tissue Diagnosis
Medulloblastoma
Attributes by original data submitter
Sample
source_name
OTX2 ChIP-seq in D341 cell line infected with CRISPR.OTX2.2
cell line
D341
chip antibody
OTX2
source type
cell line
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from formaldehyde-fixed cells (3-5 x 10^6 cells per epitope) was fragmented to a size range of 200 - 700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with the indicated antibodies and Protein G-Dynabeads (Life Technologies). Immunoprecipitated DNA was extracted with AMP Pure beads(Beckman Coulter) after crosslink reversal, RNAse A and Proteinase K treatment. ChIP DNA samples were used to generate Illumina sequencing libraries following standard protocols.