Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXA2

Cell type

Cell type Class
Epidermis
Cell type
BJ
Primary Tissue
Skin
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
FOXA2 GATA4 CoExp FOXA2 ChIP Rep1
cell line
BJFOXA2 line
source cell tyep
BJ Fibroblast
genotype/variation
Clonal FOXA2 doxycycline inducible cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were generated as previously described (Mikkelsen et al., 2010, Gifford et al., 2013) and Libraries were sequenced on a HiSeq 2500 at 11pmol.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
19524973
Reads aligned (%)
192.0
Duplicates removed (%)
2.4
Number of peaks
8962 (qval < 1E-05)

hg38

Number of total reads
19524973
Reads aligned (%)
193.5
Duplicates removed (%)
2.3
Number of peaks
9017 (qval < 1E-05)

Base call quality data from DBCLS SRA