GSM2430489: SW1783.TCF4 Wnt; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
TCF4 antibody (Cell signaling, #2569)
Sequenced DNA Library
The method for chromatin immunoprecipitation was described as ChIP assay kit (Cell Signaling Technology), while the agarose beads were replaced by magnetic beads. Indexed libraries were prepared from 6ng-20ng of Diagenode Biorupter sheared ChIP DNA using the KAPA Hyper Library Preparation Kit (Kapa Biosystems, Inc). Libraries were amplified by 6 cycles of PCR then assessed for quality using the Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analyticals) and quantified using the Qubit dsDNA HS Assay Kit (ThermoFisher).