GSM2430489: SW1783.TCF4 Wnt; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TCF4
Cell type
Cell type Class
Neural
Cell type
SW 1783
Primary Tissue
Brain
Tissue Diagnosis
Astrocytoma
Attributes by original data submitter
Sample
source_name
SW1783 cells
cell line
SW1783
treatment
Wnt treatment
antibody
TCF4 antibody (Cell signaling, #2569)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The method for chromatin immunoprecipitation was described as ChIP assay kit (Cell Signaling Technology), while the agarose beads were replaced by magnetic beads. Indexed libraries were prepared from 6ng-20ng of Diagenode Biorupter sheared ChIP DNA using the KAPA Hyper Library Preparation Kit (Kapa Biosystems, Inc). Libraries were amplified by 6 cycles of PCR then assessed for quality using the Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analyticals) and quantified using the Qubit dsDNA HS Assay Kit (ThermoFisher).