dormant bone metastatic (DBM) derivatives of T47D cells
transfection
shMSK1
chip antibody
H3K9ac (Abcam, ab4441)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with indicated antibody Libraries for sequencing were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (E7370L) following the manufacturer's instructions.