Genomic DNA was isolated and purified from homogenized liver tissue using a serial phenol: chloroform extraction. Trizol reagent was used for extracting total RNA following the manufacturers protocol. Total RNA samples are converted to Illumina sequencing libraries using Illuminas Truseq RNA Sample Preparation Kit; The final libraries from RRBS were prepared for sequencing as per the manufacturers instructions in the Illumina cBot and HiSeq Paired en