Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

Cell line

cell type

fetal brain-derived neural stem/progenitor cells

passages

11 to 20

chip antibody

AB8895

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA was isolated and purified from homogenized liver tissue using a serial phenol: chloroform extraction. Trizol reagent was used for extracting total RNA following the manufacturer’s protocol. Total RNA samples are converted to Illumina sequencing libraries using Illumina’s Truseq RNA Sample Preparation Kit; The final libraries from RRBS were prepared for sequencing as per the manufacturer’s instructions in the Illumina cBot and HiSeq Paired en

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

9517029

Reads aligned (%)

94.9

Duplicates removed (%)

5.0

Number of peaks

34 (qval < 1E-05)

hg19

Number of total reads

9517029

Reads aligned (%)

94.7

Duplicates removed (%)

5.0

Number of peaks

46 (qval < 1E-05)