Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Neutrophils

MeSH Description

Immature neutrophils.

Sample

source_name

active disease, untreated

cell type

neutrophils

disease state

Juvenile Idiopathic Arthritis

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Peripheral blood mononuclear cells (PBMC) were separated from neutrophils and red blood cells by density-gradient centrifugation. Red cells were removed from neutrophils by hypotonic lysis. Neutrophils were crosslinked using 1% formaldehyde in 10 ml PBS for 10 min at room temperature. Crosslinking was quenched by adding 1× glycine. ChIP was performed following the protocol (Cell Signaling Technologies, Inc.). DNA-sequencing was conducted using the Illumina HiSeq 2500.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

52464668

Reads aligned (%)

96.4

Duplicates removed (%)

25.4

Number of peaks

1214 (qval < 1E-05)

hg19

Number of total reads

52464668

Reads aligned (%)

95.2

Duplicates removed (%)

27.3

Number of peaks

1002 (qval < 1E-05)