Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cell line

Cell type

S2R+

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

Input for Mago HA overexpression with Mago knockdown

cell line

S2R+ Cells

chip antibody

none

cell type

Embryonic

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 µl of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA II library preparation kit, following manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

13268284

Reads aligned (%)

97.4

Duplicates removed (%)

12.2

Number of peaks

2705 (qval < 1E-05)

dm3

Number of total reads

13268284

Reads aligned (%)

97.9

Duplicates removed (%)

9.2

Number of peaks

2980 (qval < 1E-05)