GSM2422036: input DNA untreated; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
mouse embryonic fibroblasts (MEFs)
strain
C57BL/6
cell type
mouse embryonic fibroblasts (MEFs)
treatment
Untreated control on day 6
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 min, quenched with glycine and washed 2 times with PBS. Cells were then resuspended in lysis buffer and sonicated to shear the chromatin to an average length of 600 bp. Supernatants were precleared with Protein-A/G Dynabeads (Invitrogen) and 10% input was collected. Immunoprecipitations were performed with antibodies to H3K4me3 and H3K27me3 (Millipore). DNA-protein complexes were pulled down using Protein-A/G Dynabeads (Invitrogen) washed. DNA was recovered by overnight incubation at 65°C to reverse crosslinks and purified using QIAquick PCR purification columns (QIAGEN). Libraries were prepared from ChIP DNA experiments with the TruSeq® DNA LT/HT Sample Prep Kit (Illumina) following manufacturer’s instructions.