Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
NSC-derived iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
Neural stem cell (NSC)-derived pre-iPSCs
tissue
Neural stem cell (NSC)-derived pre-iPSCs
strain
129/MF1
ectopic gene
N/A
antibody
N/A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as previously described (Lee et al., 2006) ChIP-seq libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (E7370) and NEBNext Multiplex Oligos for Illumina (E7335) according to the manufacturer’s protocol. Massively parallel sequencing was performed with the Platform: Nextseq 500. Flow cell was Nextseq 500/550 High Output Flow Cell Cartridge V2, reagent was Nextseq 500/550 V2 75 cycles.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
58288214
Reads aligned (%)
88.9
Duplicates removed (%)
48.7
Number of peaks
467 (qval < 1E-05)

mm9

Number of total reads
58288214
Reads aligned (%)
88.4
Duplicates removed (%)
48.6
Number of peaks
545 (qval < 1E-05)

Base call quality data from DBCLS SRA