Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Tail bud
NA
NA

Attributes by original data submitter

Sample

source_name
Input wtE9.5Tailbud 10min fix2
tissue
E9.5 embryo tailbud
experiment type
ChIP-seq
antibody
none
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinking 10mins RT, lysis, sonication according to Amin and Bobola (2014). Illumina Truseq DNA library protocol

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
129468948
Reads aligned (%)
97.1
Duplicates removed (%)
19.1
Number of peaks
623 (qval < 1E-05)

mm9

Number of total reads
129468948
Reads aligned (%)
96.9
Duplicates removed (%)
19.0
Number of peaks
737 (qval < 1E-05)

Base call quality data from DBCLS SRA