Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD51

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS cells, vehicle, RAD51 ChIP
cell line
U2OS
cell type
sarcoma-derived cells
treatment
vehicle
chip antibody
anti-RAD51 (Santa Cruz, SC-8349, lot: G2314)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and RAD51-DNA complexes were isolated with antibody. NEBNext Ultra Library Prep Kit for Illumina.

Sequencing Platform

instrument_model
Illumina MiSeq

hg19

Number of total reads
1155922
Reads aligned (%)
64.7
Duplicates removed (%)
0.4
Number of peaks
34 (qval < 1E-05)

hg38

Number of total reads
1155922
Reads aligned (%)
65.0
Duplicates removed (%)
0.4
Number of peaks
23 (qval < 1E-05)

Base call quality data from DBCLS SRA