GSM2417776: E11.5 Mouse NT Input1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Embryo
Cell type
Neural tube
NA
NA
Attributes by original data submitter
Sample
source_name
Neural tube
developmental stage
E11.5
strain
ICR
antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For full ChIP protocol see Meredith 2013 MCB 33:3166-3179. For extraction, nuclei were liberated from neural tube tissue by dounce homogenization in PBS and then fixed in 1% formaldehyde for 10 minutes at room temperature. Fixation was terminated by adding glycine to a final concentration of 0.125M. Chromatin was sheared using a Diagenode Bioruptor for 30 minutes on high power with 30s:30s on:off cycles. 100 μg chromatin was immunoprecipitated with 5 μg rabbit anti-PRDM13 antibody (Johnson Lab generated, PA6659, rabbit anti-mouse PRDM13) followed by anti-mouse Dyna beads (Invitrogen). Libraries were made according to Illumina's ChIP-seq DNA sample prep protocol