A nutritional reservoir of fatty tissue found mainly in insects and amphibians.
Attributes by original data submitter
Sample
source_name
Male OrR late-third wandering larval fat body tissue, ORC2 ChIP
strain/background
OregonR
genotype/variation
wild-type
Sex
male
tissue
fat body
developmental stage
Late-third wandering larvae
chip antibody
anti-ORC2 (Steve Bell lab, MIT)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fat body tissue was dounced homogenized and then sonicated using a Bioruptor 300 (Diagenode) prior to ChIP with anti-ORC2 antibody. Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (Part # E7645S) according to manufacturer's recommendations with indexing primers for multiplexing.