Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Prostate
Cell type
Prostate cancer-associated fibroblasts
NA
NA

Attributes by original data submitter

Sample

source_name
Primary Fibroblast
cell type
Human prostate cancer-associated fibroblasts
passages
P5-P7
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina’s NextSeq 500 (75 nt reads, single end)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
39932108
Reads aligned (%)
91.9
Duplicates removed (%)
4.9
Number of peaks
1701 (qval < 1E-05)

hg19

Number of total reads
39932108
Reads aligned (%)
91.1
Duplicates removed (%)
6.2
Number of peaks
1291 (qval < 1E-05)

Base call quality data from DBCLS SRA