Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Muscle
Cell type
Myofibroblasts
MeSH Description
Spindle-shaped cells with characteristic CONTRACTILE PROTEINS and structures that contribute to the WOUND HEALING process. They occur in GRANULATION TISSUE and also in pathological processes such as FIBROSIS.

Attributes by original data submitter

Sample

source_name
Myofibroblast cell line
cell type
Myofibroblast cell line that overexpresses AR
passages
P5-P7
chip antibody
AR (Santa Cruz, H280, sc-13062, Lot D0414)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina’s NextSeq 500 (75 nt reads, single end)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
42450934
Reads aligned (%)
71.9
Duplicates removed (%)
19.1
Number of peaks
5261 (qval < 1E-05)

hg38

Number of total reads
42450934
Reads aligned (%)
73.2
Duplicates removed (%)
18.2
Number of peaks
5469 (qval < 1E-05)

Base call quality data from DBCLS SRA