Antigen

Antigen Class

TFs and others

Antigen

AR

Cell type

Cell type Class

Muscle

Cell type

Myofibroblasts

MeSH Description

Spindle-shaped cells with characteristic CONTRACTILE PROTEINS and structures that contribute to the WOUND HEALING process. They occur in GRANULATION TISSUE and also in pathological processes such as FIBROSIS.

Sample

source_name

Myofibroblast cell line

cell type

Myofibroblast cell line that overexpresses AR

passages

P5-P7

chip antibody

AR (Santa Cruz, H280, sc-13062, Lot D0414)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina’s NextSeq 500 (75 nt reads, single end)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

42450934

Reads aligned (%)

89.4

Duplicates removed (%)

18.2

Number of peaks

5469 (qval < 1E-05)

hg19

Number of total reads

42450934

Reads aligned (%)

88.8

Duplicates removed (%)

19.1

Number of peaks

5261 (qval < 1E-05)