Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Blood
Cell type
CUTLL1
NA
NA

Attributes by original data submitter

Sample

source_name
CUTLL1
cell type
human T cell leukemia cells
cell line
CUTLL1
antibody
MYC (Santa Cruz Biotech, N262)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1. Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (santa cruz biotechnology, N-262) against human MYC Libraries were generated as described before (Ntziachristos et al., Nat Med, 2012), including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
19581496
Reads aligned (%)
86.9
Duplicates removed (%)
9.1
Number of peaks
830 (qval < 1E-05)

hg38

Number of total reads
19581496
Reads aligned (%)
89.0
Duplicates removed (%)
7.8
Number of peaks
727 (qval < 1E-05)

Base call quality data from DBCLS SRA