GSM2411135: ChIP-Seq analysis of HSF1 in P12 cells (rep1); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
CUTLL1
NA
NA
Attributes by original data submitter
Sample
source_name
CUTLL1
cell type
human T cell leukemia cells
cell line
P12
antibody
HSF1 (Cell Signaling 4356)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1. Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (santa cruz biotechnology, N-262) against human MYC Libraries were generated as described before (Ntziachristos et al., Nat Med, 2012), including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.