Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Brain cortex
NA
NA

Attributes by original data submitter

Sample

source_name
Cortex
strain
C57BL/6
genotype
wild type
tissue
Brain Cortex
chip antibody
Input
age
6 weeks

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
total MeCP2 ChIP was performed as described in Cohen et al. Neuron 2012, Ebert et al Nature 2013 Single-end short read sequencing libraires were prepared using standard protocols, following the manufacturers instructions. Libraries were sequenced on the Illumina NextSeq ChIP-Seq was performed according to Gabel et.al., Nature 2015.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
23679987
Reads aligned (%)
97.4
Duplicates removed (%)
14.6
Number of peaks
309 (qval < 1E-05)

mm9

Number of total reads
23679987
Reads aligned (%)
97.2
Duplicates removed (%)
14.6
Number of peaks
285 (qval < 1E-05)

Base call quality data from DBCLS SRA